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Location： Home > Recombinant Rabbit Monoclonal Antibodies > ATF4 Rabbit mAb

ATF4 Rabbit mAb#49147

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Product Detail

Product NameATF4 Rabbit mAb

Clone No.SD20-92

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesActivating transcription factor 4 antibody
ATF 4 antibody
ATF4 antibody
ATF4 protein antibody
ATF4_HUMAN antibody
cAMP-dependent transcription factor ATF-4 antibody
cAMP-responsive element-binding protein 2 antibody
CREB 2 antibody
CREB-2 antibody
CREB2 antibody
Cyclic AMP dependent transcription factor ATF 4 antibody
Cyclic AMP response element binding protein 2 antibody
Cyclic AMP-dependent transcription factor ATF-4 antibody
Cyclic AMP-responsive element-binding protein 2 antibody
DNA binding protein TAXREB67 antibody
DNA-binding protein TAXREB67 antibody
Tax Responsive Enhancer Element B67 antibody
Tax-responsive enhancer element-binding protein 67 antibody
TaxREB67 antibody
TXREB antibody

Accession NoSwiss-Prot#:P18848

Uniprot P18848

Gene ID 468;

Calculated MW55 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of ATF4 on different lysates using anti-ATF4 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: PC-12
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ATF4 antibody. Counter stained with hematoxylin.
ICC staining ATF4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ATF4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ATF4 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with ATF4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promoter and enhancer elements. The cAMP response element (CRE), one of the best studied of such elements, consists of the palindromic octanucleotide TGACGTCA. Several CRE binding proteins have been identified within the ATF/CREB family, the best characterized of which include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. These proteins share highly related COOH terminal leucine zipper dimerization and basic DNA binding domains but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins appear capable of binding CRE in its homodimeric form, certain of these also bind as heterodimers, both within the ATF/CREB family and even with members of the AP-1 transcription factor family.

If you have published an article using product 49147, please notify us so that we can cite your literature.

et al,Achyranthes bidentata polysaccharides alleviate endoplasmic reticulum stress in osteoarthritis via lncRNA NEAT1/miR-377-3p pathway. In Biomed Pharmacother on 2022 Oct by Changlong Fu, Zhiwei Qiu,et al..PMID:35988424 , (2022),
PMID: 35988424
et al,The therapeutic effect of tanshinone IIA in mouse astrocytes after treatment with Angiostrongylus cantonensis fifth-stage larval excretory-secretory productsIn J Microbiol Immunol Infect.On2023 AugbyKuang-Yao Chen , Yi-Ju Chen et al..PMID:37147244 , (2023),
PMID: 37147244
et al,Peroxiredoxin 4 deficiency induces accelerated ovarian aging through destroyed proteostasis in granulosa cells. In Biochim Biophys Acta Mol Basis Dis on 2024 Oct by Xiaofei Zou, Xiuru Liang et al..PMID:38971505 , (2024),
PMID: 38971505

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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