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Location： Home > Recombinant Rabbit Monoclonal Antibodies > ATF5 Rabbit mAb

ATF5 Rabbit mAb#49148

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Product Detail

Product NameATF5 Rabbit mAb

Clone No.SD2099

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesActivating transcription factor 5 alpha/beta antibody
Activating transcription factor 5 antibody
Activating transcription factor X antibody
AFTA antibody
ATF 5 antibody
ATF 7 antibody
ATF7 antibody
ATFX antibody
BZIP protein ATF7 antibody
cAMP dependent transcription factor ATF 5 antibody
cAMP dependent transcription factor ATF5 antibody
Cyclic AMP dependent transcription factor ATF 5 antibody
Cyclic AMP dependent transcription factor ATF5 antibody
FLJ34666 antibody
HMFN0395 antibody
MGC102397 antibody
NAP1 antibody
NRIF3 associated protein antibody
ODA 10 antibody
Transcription factor ATFx antibody
Transcription factor like protein ODA 10 antibody
Transcription factor like protein ODA10 antibody

Accession NoSwiss-Prot#:Q9Y2D1

Uniprot Q9Y2D1

Gene ID 22809;

Calculated MW31 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of ATF5 on different lysates using anti-ATF5 antibody at 1/1,000 dilution. Positive control:
Lane 1: Jurkat
Lane 2: PC-12
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ATF5 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ATF5 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ATF5 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATF5 antibody. Counter stained with hematoxylin.
ICC staining ATF5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ATF5 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB3 and Luman), CREM-1, CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-5 (ATFx), which can localize to the cytoplasm or the nucleus, binds DNA as a dimer. It interacts with CCND3 and PTP4A1.

If you have published an article using product 49148, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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