Product Detail
Product NameHDAC7 Rabbit mAb
Clone No.SD082-4
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, FC
Species ReactivityHu, Ms, Rt
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesDKFZP586J0917 antibody FLJ99588 antibody HD 7a antibody HD7 antibody HD7a antibody HDAC 7 antibody HDAC 7A antibody Hdac7 antibody HDAC7_HUMAN antibody HDAC7A antibody Histone deacetylase 7 antibody Histone deacetylase 7A antibody OTTHUMP00000202813 antibody OTTHUMP00000202814 antibody
Accession NoSwiss-Prot#:Q8WUI4
Uniprot
Q8WUI4
Gene ID
51564;
Calculated MW109/99 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-1:2,000
FC: 1:50-1:100
Western blot analysis of HDAC7 on different lysates using anti-HDAC7 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Human brain
Flow cytometric analysis of K562 cells with HDAC7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC7 is a histone deacetylase that interacts with the adaptor mSin3A. The interaction of HDAC7 with mSin3A suggests the association of multiple repression complexes of transcription factors.
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