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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Cdk9 Rabbit mAb

Cdk9 Rabbit mAb#49192

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Product Detail

Product NameCdk9 Rabbit mAb

Clone No.SD204-07

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesC-2K antibody
CDC2 related kinase antibody
CDC2L4 antibody
Cdk 9 antibody
Cdk9 antibody
CDK9_HUMAN antibody
Cell division cycle 2-like protein kinase 4 antibody
Cell division protein kinase 9 antibody
CTK1 antibody
Cyclin dependent kinase 9 antibody
Cyclin-dependent kinase 9 antibody
PITALRE antibody
Serine/threonine-protein kinase PITALRE antibody
TAK antibody
Tat associated kinase complex catalytic subunit antibody

Accession NoSwiss-Prot#:P50750

Uniprot P50750

Gene ID 1025;

Calculated MW43/37 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of Cdk9 on different lysates using anti-Cdk9 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: A431
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cdk9 antibody. Counter stained with hematoxylin.
ICC staining Cdk9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk9 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
A family of proteins designated cyclin dependent kinases (Cdks) are critical regulators of cell cycle progression. Cdk family members, including Cdc2 p34, Cdk1C9, PISSLRE, KKIALRE, PITSLRE, and PCTAIRE 1C3 are constitutively expressed throughout the cell cycle. Cdc2 p34 activity peaks during mitosis and Cdk2 activity rises in late G1 or early S phase. Cdk4 and Cdk6 are critically involved in G1 to S phase progression. The functions of Cdk3, Cdk5b, PISSLRE, KKIALRE and PCTAIRE 1C3 are less well defined. Cdk9 (also designated PITALRE) has been shown to specifically phosphorylate the retinoblastoma protein. The more recently cloned Drosophila protein, P-TEFb, is thought to be the homolog of mammalian PITALRE. P-TEFb has been shown to be required for HIV Tat transcriptional activation.

If you have published an article using product 49192, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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