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HSPA14 Rabbit mAb#49209

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Product NameHSPA14 Rabbit mAb

Clone No.SD08-48

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesHeat shock 70 kDa protein 14 antibody
Heat shock 70kDa protein 14 antibody
Heat shock protein HSP60 antibody
Heat shock protein hsp70 related protein antibody
HSP60 antibody
HSP70 4 antibody
HSP70 like protein 1 antibody
HSP70-like protein 1 antibody
HSP70L1 antibody
HSP7E_HUMAN antibody
HSPA 14 antibody
HSPA14 antibody
MGC131990 antibody

Accession NoSwiss-Prot#:Q0VDF9

Uniprot Q0VDF9

Gene ID 51182;

Calculated MW55 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of HSPA14 on different lysates using anti-HSPA14 antibody at 1/1,000 dilution. Positive control:
Lane 1: K562
Lane 2: COS-1
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HSPA14 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-HSPA14 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HSPA14 antibody. Counter stained with hematoxylin.
ICC staining HSPA14 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining HSPA14 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining HSPA14 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multi-protein complexes, the transportation of nascent poly-peptide chains across cellular membranes and the regulation of protein folding. HSPA14 (heat shock 70kDa protein 14), also known as HSP70-4 or HSP70L1, is a 509 amino acid novel HSP protein derived from human dendritic cells. Belonging to the heat shock protein 70 family, HSPA14 is thought to promote dendritic cell maturation. It is also suggested that HSPA14 stimulates secretion of the proinflammatory cytokines interleukin 12p70 (IL-12p70), IL-1β, TNFα, and the chemokines IP-10, MIP-1α, MIP-1β, and normal T cell expressed and secreted (RANTES).

If you have published an article using product 49209, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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