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Location： Home > Recombinant Rabbit Monoclonal Antibodies > 14-3-3 Theta Rabbit mAb

14-3-3 Theta Rabbit mAb#49213

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Product Name14-3-3 Theta Rabbit mAb

Clone No.SD084-02

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names14-3-3 antibody
14-3-3 protein T-cell antibody
14-3-3 protein tau antibody
14-3-3 protein theta antibody
1C5 antibody
HS1 antibody
theta polypeptide antibody
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein antibody
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta isoform antibody
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide antibody

Accession NoSwiss-Prot#:P27348

Uniprot P27348

Gene ID 10971;

Calculated MW28 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000

ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of 14-3-3 Theta on different lysates using anti-14-3-3 Theta antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: MCF-7
ICC staining 14-3-3 Theta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining 14-3-3 Theta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining 14-3-3 Theta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 Theta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and . 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.

If you have published an article using product 49213, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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