Product Detail
Product NameMGMT Rabbit mAb
Clone No.JJ089-6
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, IHC
Species ReactivityHu
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other Names6 O methylguanine DNA methyltransferase antibody 6-O-methylguanine-DNA methyltransferase antibody Agat antibody AGT antibody AI267024 antibody EC 2.1.1.63 antibody Methylated DNA protein cysteine methyltransferase antibody Methylated-DNA--protein-cysteine methyltransferase antibody Methylguanine DNA methyltransferase antibody MGC107020 antibody MGMT antibody MGMT_HUMAN antibody O 6 methylguanine DNA alkyltransferase antibody O 6 methylguanine DNA methyltransferase antibody O-6-methylguanine-DNA methyltransferase antibody O-6-methylguanine-DNA-alkyltransferase antibody
Accession NoSwiss-Prot#:P16455
Uniprot
P16455
Gene ID
4255;
Calculated MW22 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
Western blot analysis of MGMT on different lysates using anti-MGMT antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGMT antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MGMT antibody. Counter stained with hematoxylin.
MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation, only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, high MGMT activity was associated with the presence of mutant p53, in a study of oral cancer cell lines. Similarly, MGMT activity was significantly lower in ovarian tumors with wildtype p53 than in tumors with mutant p53, supporting the view that wildtype p53 down-regulates the basal MGMT promoter.
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