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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Integrin alpha 5 Rabbit mAb

Integrin alpha 5 Rabbit mAb#49269

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Product Detail

Product NameIntegrin alpha 5 Rabbit mAb

Clone No.SR4534

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB IHC ICC/IF

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCD49 antigen-like family member E antibody
CD49e antibody
Fibronectin receptor subunit alpha antibody
Fibronectin receptor, alpha subunit antibody
FNRA antibody
Integrin alpha 5 (fibronectin receptor alpha) antibody
Integrin alpha-5 antibody
Integrin alpha-5 light chain antibody
Integrin alpha-F antibody
Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody
ITA5_HUMAN antibody
Itga5 antibody
Very late activation protein 5, alpha subunit antibody
VLA-5 antibody
VLA5 antibody
VLA5A antibody

Accession NoSwiss-Prot#:P08648

Uniprot P08648

Gene ID 3678;

Calculated MWPredicted band size: 115 kDa

Sdspage MWObserved band size: 150 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Integrin alpha 5 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : 3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 115 kDa
Observed band size: 150 kDa

Exposure time: 7 seconds
All lanes :Integrin alpha 5 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : Integrin alpha 5 knockdown HAP1 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Integrin alpha 5 using 49269 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse uterus tissue stained for Integrin alpha 5 using 49269 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Integrin alpha 5 antibody (49269)

ICC/IF staining of Integrin alpha 5 in A549 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 49269 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

Integrins are heterodimers composed of noncovalently associated transmembrane α and β subunits. The 16 α and 8 β subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.

If you have published an article using product 49269, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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