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COX IV Rabbit mAb#49275

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Product Detail

Product NameCOX IV Rabbit mAb

Clone No.JJ09-05

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAL024441 antibody
COX 4 antibody
COX IV 1 antibody
COX IV antibody
COX IV-1 antibody
Cox4 antibody
COX41_HUMAN antibody
Cox4a antibody
COX4B antibody
COX4I1 antibody
COX4I2 antibody
COX4L2 antibody
COXIV antibody
Cytochrome c oxidase polypeptide IV antibody
Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
Cytochrome C Oxidase subunit IV antibody
Cytochrome c oxidase subunit IV isoform 1 antibody
Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
Cytochrome c oxydase subunit 4 antibody
dJ857M17.2 antibody
MGC105470 antibody
MGC72016 antibody

Accession NoSwiss-Prot#:P13073

Uniprot P13073

Gene ID 1327;

Calculated MW16 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of COX IV on different lysates using anti-COX IV antibody at 1/1,000 dilution. Positive control:
Lane 1: Mouse heart
Lane 2: Rat heart
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-COX IV antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-COX IV antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-COX IV antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-COX IV antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-COX IV antibody. Counter stained with hematoxylin.
ICC staining COX IV in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining COX IV in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of MCF-7 cells with COX IV antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Cytochrome c oxidase (COX) functions as the terminal oxidase of the respiratory chain that uses cytochrome c as an electron donor to drive a proton gradient across the inner mitochondrial membrane. The mammalian COX apoenzyme is a heteromer consisting of three mitochondrial encoded catalytic subunits and several nuclear gene encoded structural subunits. COX contains two iron-coordination sites and two copper-coordination sites. Cytochrome c oxidase IV (COX4) is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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