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FOXO4 Rabbit mAb#49279

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Product NameFOXO4 Rabbit mAb

Clone No.JJ09-11

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAFX antibody
AFX1 antibody
Afxh antibody
ALL1-fused gene from X chromosome antibody
Fork head domain transcription factor AFX1 antibody
Forkhead box O4 antibody
Forkhead box protein O4 antibody
FOXO 4 antibody
Foxo4 antibody
FOXO4_HUMAN antibody
MGC117660 antibody
MGC120490 antibody
Mixed lineage leukemia, translocated to, 7 antibody
MLLT7 antibody
Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 antibody
Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7 antibody
RGD1561201 antibody

Accession NoSwiss-Prot#:P98177

Uniprot P98177

Gene ID 4303;

Calculated MW54 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000

ICC: 1:100-1:500

FC: 1:50-1:100
ICC staining FOXO4 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining FOXO4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining FOXO4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of 293 cells with FOXO4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
FKHR (for forkhead in rhabdomyosarcoma), FKHRL1, and AFX1 are members of a subfamily of the forkhead family of transcription factors. AFX1, also known as FoxO4, is expressed in a wide variety of tissues and, like other FKHR proteins, AFX1 contains a single forkhead domain and serine-proline-rich region, which mediate DNA binding. AFX1-mediated transcriptional activation is regulated by the serine/threonine kinase Akt1, which phosphorylates AFX1 and in turn, sequesters AFX1 in the cytosol, thereby blocking nuclear localization and DNA binding. Genetic mutations in FKHR genes, including the t(2;13) and t(1;3) translocations, are commonly found in alveolar rhabdomyosarcomas. Additionally, the t(x;11) translocation of the AFX1 gene, which involves the fusion of a serine-proline-rich sequence of AFX1 to the carboxy terminus of a truncated MLL, results in acute lymphocytic leukemia.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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