Product Detail
Product NameRIP Rabbit mAb
Clone No.JJ092-08
Host SpeciesRecombinant Rabbit
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, FC
Species ReactivityHu
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesCell death protein RIP antibody FLJ39204 antibody OTTHUMP00000039163 antibody Receptor (TNFRSF) interacting serine threonine kinase 1 antibody receptor interacting protein 1 antibody Receptor interacting protein antibody Receptor interacting protein kinase 1 antibody Receptor interacting serine threonine protein kinase 1 antibody Receptor TNFRSF interacting serine threonine kinase 1 antibody Receptor-interacting protein 1 antibody Receptor-interacting serine/threonine-protein kinase 1 antibody Rinp antibody RIP 1 antibody RIP antibody Rip-1 antibody RIP1 antibody RIPK 1 antibody Ripk1 antibody RIPK1_HUMAN antibody Serine threonine protein kinase RIP antibody Serine/threonine-protein kinase RIP antibody
Accession NoSwiss-Prot#:Q13546
Uniprot
Q13546
Gene ID
8737;
Calculated MW76 kDa
Sdspage MW76 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-5,000
FC: 1:50-1:100
Western blot analysis of RIP on Hela cells lysates using anti-RIP antibody at 1/1,000 dilution.
Flow cytometric analysis of 293 cells with RIP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
All lanes : RIP Rabbit mAb at 1/1k dilution Lane 1 : HEK293 cell lysate Lane 2 : Hela cell lysate Lane 3 : Jurkat cell lysate Lane 4 : Raji cell lysate Lysates/proteins at 20 µg per lane. Predicted band size: 76kDa Observed band size: 76kDa
All lanes : RIP Rabbit mAb at 1/1k dilution Lane 1 : Wild-type HAP1 cell lysate Lane 2 : RIP knockout HAP1 cell lysate Lysates/proteins at 20 µg per lane.
In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved "death domain" and belonging to the TNF receptor superfamily. TRADD, FADD and RIP are FAS/TNF-R1 interacting proteins that contain a death domain homologous region (DDH). TRADD (TNF-R1-associated death domain) and FADD (FAS-associated death domain) associate with the death domains of both FAS and TNF-R1 via their DDH regions. Overexpression of TRADD leads to NFkB activation and apoptosis in the absence of TNF. Overexpression of FADD causes apoptosis, which can be blocked by the cow pox protein CrmA, suggesting that FADD lies upstream of ICE and possibly other serine proteases. The receptor interacting protein, RIP, associates with FAS exclusively via its DDH and this association is abrogated in lpr mutants. Unlike TRADD and FADD, RIP contains a putative amino terminal kinase domain.
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