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Bmi1 Rabbit mAb#49303

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Product Detail

Product NameBmi1 Rabbit mAb

Clone No.JJ093-3

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB IHC ICC/IF

Species ReactivityHu, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesB lymphoma Mo MLV insertion region (mouse) antibody
B lymphoma Mo MLV insertion region 1 homolog antibody
Bmi 1 antibody
BMI1 antibody
BMI1 polycomb ring finger oncogene antibody
BMI1_HUMAN antibody
Flvi 2/bmi 1 antibody
FLVI2/BMI1 antibody
MGC12685 antibody
Murine leukemia viral (bmi 1) oncogene homolog antibody
Oncogene BMI 1 antibody
PCGF 4 antibody
PCGF4 antibody
Polycomb complex protein BMI 1 antibody
Polycomb complex protein BMI-1 antibody
Polycomb group protein Bmi1 antibody
Polycomb group ring finger 4 antibody
Polycomb group RING finger protein 4 antibody
RING finger protein 51 antibody
RNF 51 antibody
RNF51 antibody

Accession NoSwiss-Prot#:P35226

Uniprot P35226

Gene ID 100532731;648;

Calculated MWPredicted band size: 37 kDa

Sdspage MWObserved band size: 42 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Bmi1 Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : 293 whole cell lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 37 kDa
Observed band size: 42 kDa

Exposure time: 5 seconds
All lanes:Bmi1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type 293T cell lysate
Lane 2 : Bmi1 knockdown 293T cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Bmi1 using 49303 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Bmi1 using 49303 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat smooth muscle tissue stained for Bmi1 using 49303 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Bmi1 antibody (49303)

ICC/IF staining of Bmi1 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 49303 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

In Drosophila, Polycomb (Pc-g) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. Mel-18 and Bmi-1, mammalian homologs of Drosophila Pc-g group proteins, are similarly expressed during development and implicated in the regulation of gene expression, axial skeleton development, control of proliferation and survival of haematopoietic cells. Mel-18 directly binds to DNA through a RING-finger motif and preferentially associates with juxtaposed enhancer elements on various genes, including Bcl-2, c-Myc and Hox. Mel-18 is an immediate early response gene within the c-Myc/Cdc25 signaling cascade that exhibits tumor suppressor activity and negatively regulates cell cycle progression by blocking S phase entry. Alternatively, Bmi-1 has been identified as a potent oncogene as it contributes to the transcriptional activation of genes implicated in early lymphoid development. Proviral activation of Bmi-1 expression corresponds to enhanced gene-specific activation of other proto-oncogenes, including c-Myc and Pim, subsequently resulting in the progression of lymphomagenesis.

If you have published an article using product 49303, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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