Product Detail
Product NameEBP50 Rabbit mAb
Clone No.JJ0946
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, IHC
Species ReactivityHu, Rt
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesEBP 50 antibody EBP50 antibody Ezrin radixin moesin binding phosphoprotein 50 antibody Ezrin-radixin-moesin-binding phosphoprotein 50 antibody Na(+)/H(+) exchange regulatory cofactor NHE RF antibody Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody Na+/H+ exchange regulatory co factor antibody NHERF 1 antibody NHERF antibody NHERF-1 antibody NHERF1 antibody NHRF1_HUMAN antibody NPHLOP2 antibody Regulatory cofactor of Na(+)/H(+) exchanger antibody Slc9a3r1 antibody Sodium hydrogen exchanger regulatory factor 1 antibody Sodium-hydrogen exchanger regulatory factor 1 antibody Sodium/hydrogen exchanger regulatory factor 1 antibody Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody Solute carrier family 9 isoform 3 regulatory factor 1 antibody Solute carrier family 9 isoform A3 regulatory factor 1 antibody Solute carrier family 9 member 3 regulator 1 antibody
Accession NoSwiss-Prot#:O14745
Uniprot
O14745
Gene ID
9368;
Calculated MW50 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
Western blot analysis of EBP50 on MCF-7 cells lysates using anti-EBP50 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.
The Na+/H+ exchange protein (NHE3) functions in transepithelial Na+ absorption and is primarily expressed in the intestinal and renal brush border membrane. NHE3 regulatory factor 1 (NHERF-1) interacts with NHE3 through two PDZ (for PSD-95, Discs-large and ZO-1 homology) domains, which are protein-protein interaction modules that associate with specific carboxy-terminal motifs on target proteins. Also known as EBP50, NHERF-1 facilitates cAMP inhibition of NHE3 to decrease Na+ adsorption. NHERF-1 functions as a scaffold for an essential multiprotein complex of Ezrin and NHE3 for cAMP-mediated phosphorylation and consequent inhibition of NHE3. The amino-terminal PDZ domain regulates the dimerization of NHERF-1 in vivo. G protein-coupled receptor kinase 6A phosphorylates NHERF-1 at Ser 289 via a PDZ domain-mediated interaction. NHERF-2, also known as E3KARP, is a ubiquitously expressed protein which also functions in NHE2 regulation.
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