Product Detail
Product NameLaminnin 5 alpha 3 Rabbit mAb
Clone No.JJ0957
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF
Species ReactivityHu
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesE170 antibody Epiligrin 170 kDa subunit antibody Epiligrin subunit alpha antibody Kalinin subunit alpha antibody LAMA3 antibody LAMA3A antibody Laminin 5 alpha3 antibody Laminin A3 antibody laminin alpha 3 antibody laminin alpha3 antibody Laminin subunit alpha-3 antibody Laminin-5 subunit alpha antibody Laminin-6 subunit alpha antibody Laminin-7 subunit alpha antibody laminin5 alpha 3 antibody Laminin5 alpha3 antibody LAMNA antibody LOCS antibody Nicein subunit alpha antibody
Accession NoSwiss-Prot#:Q16787
Uniprot
Q16787
Gene ID
3909;
Calculated MW367 kDa
Concentration0.6 mg/ml
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-1:2,000
ICC: 1:100-1:500
Western blot analysis of Laminnin 5 alpha 3 on A431 cells lysates using anti-Laminnin 5 alpha 3 antibody at 1/1,000 dilution.
ICC staining Laminnin 5 alpha 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Laminnin 5 alpha 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival. Laminin alpha 3/Laminin-5, also known as epiligrin, includes alpha 3, beta 3, and gamma 2 subunits. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and contributes to initiation and maintenance of epithelial cell anchorage to the underlying connective tissue. Within aa 21?1713 of the alpha 3 subunit, human and mouse share 77% amino acid sequence identity.