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Laminnin 5 alpha 3 Rabbit mAb#49314

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Product Detail

Product NameLaminnin 5 alpha 3 Rabbit mAb

Clone No.JJ0957

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesE170 antibody
Epiligrin 170 kDa subunit antibody
Epiligrin subunit alpha antibody
Kalinin subunit alpha antibody
LAMA3 antibody
LAMA3A antibody
Laminin 5 alpha3 antibody
Laminin A3 antibody
laminin alpha 3 antibody
laminin alpha3 antibody
Laminin subunit alpha-3 antibody
Laminin-5 subunit alpha antibody
Laminin-6 subunit alpha antibody
Laminin-7 subunit alpha antibody
laminin5 alpha 3 antibody
Laminin5 alpha3 antibody
LAMNA antibody
LOCS antibody
Nicein subunit alpha antibody

Accession NoSwiss-Prot#:Q16787

Uniprot Q16787

Gene ID 3909;

Calculated MW367 kDa

Concentration0.6 mg/ml

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000-1:2,000

ICC: 1:100-1:500

Western blot analysis of Laminnin 5 alpha 3 on A431 cells lysates using anti-Laminnin 5 alpha 3 antibody at 1/1,000 dilution.
ICC staining Laminnin 5 alpha 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Laminnin 5 alpha 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival. Laminin alpha 3/Laminin-5, also known as epiligrin, includes alpha 3, beta 3, and gamma 2 subunits. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and contributes to initiation and maintenance of epithelial cell anchorage to the underlying connective tissue. Within aa 21?1713 of the alpha 3 subunit, human and mouse share 77% amino acid sequence identity.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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