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SP1 Rabbit mAb#49316

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Product Detail

Product NameSP1 Rabbit mAb

Clone No.JF0950

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu

Immunogen DescA synthesized peptide derived from human SP1

ConjugateUnconjugated

Other NamesSP 1 antibody
SP1 antibody
Sp1 transcription factor antibody
SP1_HUMAN antibody
Specificity protein 1 antibody
Transcription factor Sp1 antibody
TSFP 1 antibody
TSFP1 antibody

Accession NoSwiss-Prot#:P08047

Uniprot P08047

Gene ID 6667;

Calculated MW81 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB 1:1000-1:2000;
IHC 1:100-1:200;
ICC/IF 1:50-1:200
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-SP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SP1 antibody. Counter stained with hematoxylin.
ICC staining SP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining SP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining SP1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21-bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A), may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.1.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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