Product Detail
Product NameTelomerase reverse transcriptase Rabbit mAb
Clone No.JF0586
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, FC
Species ReactivityHu
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesCMM9 antibody DKCA2 antibody DKCB4 antibody EST2 antibody HEST2 antibody htert antibody hTRT antibody PFBMFT1 antibody TCS1 antibody Telomerase associated protein 2 antibody Telomerase catalytic subunit antibody Telomerase reverse transcriptase antibody Telomerase-associated protein 2 antibody Telomere Reverse Transcriptase antibody TERT antibody TERT_HUMAN antibody TP2 antibody TRT antibody
Accession NoSwiss-Prot#:O14746
Uniprot
O14746
Gene ID
7015;
Calculated MW127/120/90/89 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-1:2,000
FC: 1:50-1:100
Western blot analysis of TERT on Hela cells lysates using anti-TERT antibody at 1/1,000 dilution.
Flow cytometric analysis of Hela cells with TERT antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Telomerase is an RNA-dependent DNA polymerase that catalyzes the addition of telomeric repeat sequences to chromosome ends. In most human somatic cells, telomerase activity is undetectable, and telomeres shorten with successive cell divisions. However, telomerase activity is detectable in immortal cells and in many human tumors. Two candidate mammalian telomerase proteins have been cloned. Human TP1 (for telomerase-associated protein 1), also designated TLP1 in rat (for telomerase protein component 1), is homologous to the Tetrahymena p80 telomerase protein and has been shown to interact with mammalian telomerase RNA. Human TERT (for telomerase reverse transcriptase), also designated hEST2 (for ever shorter telomeres), is homologous to the p123 telomerase protein from Euplotes and to the yeast Est2 protein. Expression of TERT mRNA has been shown to correlate with telomerase activity in various cell lines.
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