Product Detail
Product NameApolipoprotein A1 Rabbit mAb
Clone No.JF0548
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF, IHC, IP
Species ReactivityHu, Ms
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesApo-AI antibody ApoA I antibody ApoA-I antibody APOA1 antibody APOA1_HUMAN antibody Apolipoprotein A-I(1-242) antibody Apolipoprotein A1 antibody Apolipoprotein AI antibody Brp14 antibody Ltw1 antibody Lvtw1 antibody Sep1 antibody Sep2 antibody
Accession NoSwiss-Prot#:P02647
Uniprot
P02647
Gene ID
335;
Calculated MW31 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Apolipoprotein A1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Apolipoprotein A1 antibody. Counter stained with hematoxylin.
ICC staining Apolipoprotein A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Apolipoprotein A1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Apolipoprotein A1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Apolipoproteins are protein components of plasma lipoproteins. The human apoA-I gene encodes a single chain, 243 amino acid protein which promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. It can function as a cofactor for lecithin cholesterolacyltransferase (LCAT), which is responsible for the formation of most plasma cholesteryl esters. The human apoA-II gene encodes the second most abundant protein of HDL particles, where it influences plasma levels of free fatty acids (FFA). The human apoA-IV gene encodes a 396 amino acid preprotein, which after proteolytic processing is secreted from the intestine in association with chylomicron particles. ApoA-IV is a potent activator of LCAT in vitro. The human apoA-V gene encodes a 366 amino acid protein that is believed to be an important determinant of plasma triglyceride levels.
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