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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Fibronectin Rabbit mAb

Fibronectin Rabbit mAb#49339

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Product Detail

Product NameFibronectin Rabbit mAb

Clone No.JF0582

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesCIG antibody
Cold insoluble globulin antibody
Cold-insoluble globulin antibody
DKFZp686F10164 antibody
DKFZp686H0342 antibody
DKFZp686I1370 antibody
DKFZp686O13149 antibody
ED B antibody
Fibronectin 1 antibody
FINC antibody
FINC_HUMAN antibody
FN antibody
FN1 antibody
FNZ antibody
GFND antibody
GFND2 antibody
LETS antibody
Migration stimulating factor antibody
MSF antibody
Ugl-Y3 antibody

Accession NoSwiss-Prot#:P02751

Uniprot P02751

Gene ID 2335;

Calculated MW263 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:100-1:500

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Fibronectin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Fibronectin antibody. Counter stained with hematoxylin.
ICC staining Fibronectin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Fibronectin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Fibronectin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblast phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren's disease.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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