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Location： Home > Recombinant Rabbit Monoclonal Antibodies > S100 alpha 6 Rabbit mAb

S100 alpha 6 Rabbit mAb#49342

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Product Detail

Product NameS100 alpha 6 Rabbit mAb

Clone No.JF0976

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names2A9 antibody
5B10 antibody
CABP antibody
CACY antibody
Calcyclin antibody
Growth factor inducible protein 2A9 antibody
Growth factor-inducible protein 2A9 antibody
MLN 4 antibody
MLN4 antibody
OTTHUMP00000015472 antibody
OTTHUMP00000015473 antibody
PRA antibody
PRAGrowth factor inducible protein 2A9 antibody
Prolactin receptor associated protein antibody
Prolactin receptor-associated protein antibody
Protein S100 A6 antibody
Protein S100-A6 antibody
S100 A6 antibody
S100 calcium binding protein A6 (calcyclin) antibody
S100 calcium binding protein A6 antibody
S100 calcium-binding protein A6 antibody
S100A6 antibody
S10A6_HUMAN antibody

Accession NoSwiss-Prot#:P06703

Uniprot P06703

Gene ID 6277;

Calculated MW10 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of S100 alpha 6 on different lysates using anti-S100 alpha 6 antibody at 1/1,000 dilution. Positive control:
Lane 1: A431
Lane 2: Mouse lung
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.
ICC staining S100 alpha 6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining S100 alpha 6 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining S100 alpha 6 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY-5Y cells with S100 alpha 6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Calcyclin, also known as Prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9, S-100 calcium-binding protein A6 (S-100A6) or MLN 4, is a homodimeric member of the S-100 calcium-binding protein family whose expression is upregulated in proliferating and differentiating cells. Calcyclin is inducible by growth factors and overexpressed in acute myeloid leukemias. It is expressed in a cell-specific manner in subpopulations of neurons and astrocytes and in epithelial cells and fibroblasts. Calcyclin is a specific target of S-100B protein in vivo. The binding of Calcyclin to S-100B is stabilized by S-100B-bound calcium and zinc. Calcyclin associates with both Annexin XI and CacyBP (calcyclin-binding protein). It functions to activate several processes along the calcium signal transduction pathway including the regulation of cell growth, proliferation, secretion and exocytosis.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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