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Location： Home > Recombinant Rabbit Monoclonal Antibodies > gamma Tubulin Rabbit mAb

gamma Tubulin Rabbit mAb#49346

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Product Detail

Product Namegamma Tubulin Rabbit mAb

Clone No.JF0972

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms, Rt, Monkey, Chicken, Hamster, Cow, Dog, Fish, Xenopus tropicalis

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesGamma 1 tubulin antibody
Gamma 2 tubulin antibody
Gamma Tubulin 1 antibody
Gamma Tubulin 2 antibody
Gamma tubulin complex component 1 antibody
Gamma-2-tubulin antibody
GCP 1 antibody
GCP1 antibody
MGC131994 antibody
TBG2_HUMAN antibody
TUBG antibody
TUBG1 antibody
TUBG2 antibody
TUBGCP1 antibody
Tubulin gamma 1 chain antibody
Tubulin gamma 2 chain antibody
Tubulin gamma complex-associated protein 1 antibody
Tubulin gamma-2 chain antibody
tubulin, gamma 1 antibody
tubulin, gamma 2 antibody
tubulin, gamma polypeptide antibody
Xgam antibody

Accession NoSwiss-Prot#:P23258

Uniprot P23258

Gene ID 7283;

Calculated MW51 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of gamma Tubulin on different lysates using anti-gamma Tubulin antibody at 1/1,000 dilution. Positive control:
Lane 1: A431
Lane 2: Jurkat
Lane 3: Mouse brain
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-gamma Tubulin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-gamma Tubulin antibody. Counter stained with hematoxylin.
ICC staining gamma Tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining gamma Tubulin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining gamma Tubulin in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Jurkat cells with gamma Tubulin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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