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Dysferlin Rabbit mAb#49359

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Product Detail

Product NameDysferlin Rabbit mAb

Clone No.JF05-54

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms

Immunogen DescA synthesized peptide derived from human Dysferlin

ConjugateUnconjugated

Other NamesDMAT antibody
DYSF antibody
DYSF_HUMAN antibody
Dysferlin antibody
Dysferlin limb girdle muscular dystrophy 2B (autosomal recessive) antibody
Dysferlin limb girdle muscular dystrophy 2B antibody
Dystrophy associated fer 1 like 1 antibody
Dystrophy associated fer 1 like protein antibody
Dystrophy associated fer1 like 1 antibody
Dystrophy associated fer1 like protein antibody
Dystrophy-associated fer-1-like protein antibody
Fer 1 like protein 1 antibody
Fer-1-like protein 1 antibody
Fer1 like protein 1 antibody
FER1L1 antibody
FLJ00175 antibody
FLJ90168 antibody
LGMD 2B antibody
LGMD2B antibody
Limb girdle muscular dystrophy 2B (autosomal recessive) antibody
Limb girdle muscular dystrophy 2B antibody
Miyoshi myopathy antibody
MM antibody
MMD1 antibody

Accession NoSwiss-Prot#:O75923

Uniprot O75923

Gene ID 8291;

Calculated MW237 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Application Details

WB 1:1000-1:2000
IHC 1:100-1:200
ICC/IF 1:50-1:200

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.
ICC staining Dysferlin in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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