Product Detail
Product NameMHC class I Rabbit mAb
Clone No.JF10-38
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF, IHC, FC
Species ReactivityHu
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesA 28 antibody
A 9 antibody
Antigen presenting molecule antibody
Aw 24 antibody
Aw 68 antibody
CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
H2 K1 antibody
H2K antibody
HLA A antibody
HLA class I histocompatibility antigen A 1 alpha chain antibody
HLA class I histocompatibility antigen A 11 alpha chain antibody
HLA class I histocompatibility antigen A 2 alpha chain antibody
HLA class I histocompatibility antigen A 24 alpha chain antibody
HLA class I histocompatibility antigen A 3 alpha chain antibody
HLA class I histocompatibility antigen A 30 alpha chain antibody
HLA class I histocompatibility antigen A 32 alpha chain antibody
HLA class I histocompatibility antigen A 68 alpha chain antibody
HLAA antibody
Leukocyte antigen class I A antibody
Major histocompatibility complex class I A antibody
MHC class I antigen A 1 antibody
MHC class I antigen A*11 antibody
MHC class I antigen A*2 antibody
MHC class I antigen A*24 antibody
MHC class I antigen A*3 antibody
MHC class I antigen A*30 antibody
MHC class I antigen A*32 antibody
MHC class I antigen A*68 antibody
MHC class I antigen HLA A heavy chain antibody
MHC class I heavy chain H2 K antibody
Accession NoSwiss-Prot:P30443
Gene ID:3105
Uniprot
P04439
Gene ID
3105;
Calculated MW41 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC: 1:50-1:100
Western blot analysis of MHC class I on different lysates using anti-MHC class I antibody at 1/1,000 dilution. Positive control:
Lane 1: Raji
Lane 2: THP-1
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MHC class I antibody. Counter stained with hematoxylin.
ICC staining MHC class I in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of THP-1 cells with MHC class I antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an a or heavy chain, and β-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an a and b chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
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