Product Detail
Product NameGalectin 3 Rabbit mAb
Clone No.JF39-10
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF, FC
Species ReactivityHu, Rt
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other Names35 kDa lectin antibody Carbohydrate binding protein 35 antibody Carbohydrate-binding protein 35 antibody CBP 35 antibody CBP35 antibody Gal-3 antibody GAL3 antibody Galactose-specific lectin 3 antibody Galactoside-binding protein antibody GALBP antibody Galectin 3 internal gene,included antibody Galectin-3 antibody Galectin3 antibody GALIG antibody GBP antibody IgE binding protein antibody IgE-binding protein antibody L 31 antibody L 34 antibody L-31 antibody L-34 galactoside-binding lectin antibody L31 antibody Laminin-binding protein antibody Lectin L-29 antibody Lectin, galactose binding, soluble 3 antibody LEG3_HUMAN antibody LGALS2 antibody LGALS3 antibody MAC 2 antigen antibody Mac-2 antibody Mac-2 antigen antibody MAC2 antibody Macrophage galactose-specific lectin antibody MGC105387 antibody
Accession NoSwiss-Prot#:P17931
Uniprot
P17931
Gene ID
3958;
Calculated MW26 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-5,000
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of Galectin 3 on different lysates using anti-Galectin 3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: THP-1
ICC staining Galectin 3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Galectin 3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Galectin 3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Galectins are a family of soluble b-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) inter- actions and play a role in tumor progression, pre-mRNA splicing and apop-tosis. The galectin-3 protein, also known as Mac-2, hMac-2, GALBP, CBP35 or LGALS3, contains a single carbohydrate binding domain, which binds galactose-containing glycoconjugates. Galectin-3 is expressed in colonic and intestinal epithelium, inflammatory macrophages, papillary and follicular carcinomas, neoplastic astrocytes and some B and T lymphocytes. Upregulated expression of galectin-3 is involved in cancer progression and metastasis. Galectin-3 mediates the endocytosis of β1 Integrins in a lactose-dependent manner and is associated with thyroid malignancy and Crohn's disease. It may also be used as a marker for diagnosing cases involving Hurthle cell adenomas and carcinomas.
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