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PDGFR alpha Rabbit mAb#49363

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Product Detail

Product NamePDGFR alpha Rabbit mAb

Clone No.JF104-6

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAlpha-type platelet-derived growth factor receptor antibody
CD140 antigen-like family member A antibody
CD140a antibody
CD140a antigen antibody
MGC74795 antibody
PDGF alpha chain antibody
PDGF-R-alpha antibody
PDGFR 2 antibody
PDGFR alpha antibody
PDGFR2 antibody
PDGFRA antibody
PDGFRA/BCR fusion antibody
PGFRA_HUMAN antibody
Platelet derived growth factor receptor 2 antibody
Platelet derived growth factor receptor alpha antibody
Platelet derived growth factor receptor alpha polypeptide antibody
Platelet derived growth factor receptor antibody
Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
RHEPDGFRA antibody

Accession NoSwiss-Prot#:P16234

Uniprot P16234

Gene ID 5156;

Calculated MW123 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of PDGFR alpha on NIH/3T3 cells lysates using anti-PDGFR alpha antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin.
ICC staining PDGFR alpha in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PDGFR alpha in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with PDGFR alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.


Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-α and -β, which are endowed with protein tyrosine kinase domains. PDGFR-α can bind to both A and B subunits of PDGF, while PDGFR-β can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two α receptors, PDGF-AB induces dimerization of αα and αβ and PDGF-BB induces the formation of three types of dimers, αα, αβ and ββ. Translocation of the PDGFR-β gene with the Tel gene is linked to chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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