Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Recombinant Rabbit Monoclonal Antibodies > CD74 Rabbit mAb

CD74 Rabbit mAb#49365

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameCD74 Rabbit mAb

Clone No.JF40-10

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu

Immunogen DescA synthesized peptide derived from human CD74

ConjugateUnconjugated

Other NamesCD 74 antibody
CD74 antibody
CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
CD74 antigen antibody
CD74 molecule antibody
CD74 molecule, major histocompatibility complex, class II invariant chain antibody
CLIP antibody
DHLAG antibody
Gamma chain of class II antigens antibody
HG2A_HUMAN antibody
HLA class II histocompatibility antigen gamma chain antibody
HLA DR antigens associated invariant chain antibody
HLA DR gamma antibody
HLA-DR antigens-associated invariant chain antibody
HLA-DR-gamma antibody
HLADG antibody
HLADR antigens associated invariant chain antibody
Ia antigen associated invariant chain antibody
Ia antigen-associated invariant chain antibody
Ia associated invariant chain antibody
Ia gamma antibody
Ii antibody
Invariant polypeptide of major histocompatibility complex class II antigen associated antibody
la-gamma antibody
Major histocompatibility complex class II invariant chain antibody
MHC HLA DR gamma chain antibody
MHC HLA-DR gamma chain antibody
p33 antibody
p35 antibody
Protein 41 antibody

Accession NoSwiss-Prot#:P04233

Uniprot P04233

Gene ID 972;

Calculated MW34 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Application Details

WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100

Western blot analysis of CD74 on Raji cells lysates using anti-CD74 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD74 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD74 antibody. Counter stained with hematoxylin.
ICC staining CD74 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD74 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD74 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Jurkat cells with CD74 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

The human histocompatibility leukocyte antigen (HLA) class II-associated invariant chain is composed of at least four polypeptides. One of these polypeptide chains is expressed as a membrane-bound subunit and has been designated CD74. The loading of peptide onto the class II MHC protein (MHC II) appears to be regulated by CD74, which associates with MHC II during its migration to the endosomal compartment, where class II binds pep-tide. CD74 is expressed by cells of both T lymphocyte and B lymphocyte lineages. In fact, CD74 is broadly expressed in normal B lymphocytes, regardless of their histocompatibility leukocyte antigen (HLA) phenotype, while a subset of peripheral T lymphocytes that are MHC II negative do not express CD74.

If you have published an article using product 49365, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors