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CD147 Rabbit mAb#49372

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Product Detail

Product NameCD147 Rabbit mAb

Clone No.JF1-045

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names5A11 antigen antibody
5F7 antibody
BASI_HUMAN antibody
Basigin (Ok blood group) antibody
Basigin antibody
Blood brain barrier HT7 antigen antibody
Bsg antibody
CD 147 antibody
CD147 antibody
CD147 antigen antibody
Collagenase stimulatory factor antibody
EMMPRIN antibody
Extracellular matrix metalloproteinase inducer antibody
Leukocyte activation antigen M6 antibody
M 6 antibody
M6 antibody
M6 leukocyte activation antigen antibody
Neurothelin antibody
OK antibody
OK blood group antibody
OK blood group antigen antibody
TCSF antibody
Tumor cell derived collagenase stimulatory factor antibody
Tumor cell-derived collagenase stimulatory factor antibody

Accession NoSwiss-Prot#:P35613

Uniprot P35613

Gene ID 682;

Calculated MW42 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of CD147 on different lysates using anti-CD147 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: Mouse kidney
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD147 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD147 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CD147 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD147 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CD147 antibody. Counter stained with hematoxylin.
ICC staining CD147 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD147 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD147 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Extracellular matrix metalloproteinase inducer (EMMPRIN), also designated basigin or CD147, is involved in the regulation of matrix remodeling at the epidermal-dermal interface. EMMPRIN stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes, which are typically increased during tissue degradation and wound healing, are important factors in cancer invasion and metastasis.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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