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Location： Home > Recombinant Rabbit Monoclonal Antibodies > HE4 Rabbit mAb

HE4 Rabbit mAb#49375

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Product Detail

Product NameHE4 Rabbit mAb

Clone No.JF62-09

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, IP, FC

Species ReactivityHu, Ms

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesdJ461P17.6 antibody
EDDM4 antibody
epididymal protein 4 antibody
Epididymal secretory protein E4 antibody
Epididymis specific whey acidic protein type four disulfide core antibody
HE 4 antibody
Major epididymis specific protein E4 antibody
Major epididymis-specific protein E4 antibody
MGC57529 antibody
Putative protease inhibitor WAP5 antibody
WAP 5 antibody
WAP domain containing protein HE4 antibody
WAP domain containing protein HE4 V4 antibody
WAP four disulfide core domain 2 antibody
WAP four disulfide core domain protein 2 antibody
WAP four-disulfide core domain protein 2 antibody
WAP5 antibody
WFDC 2 antibody
WFDC2 antibody
WFDC2_HUMAN antibody

Accession NoSwiss-Prot#:Q14508

Uniprot Q14508

Gene ID 10406;

Calculated MW23 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of HE4 on different lysates using anti-HE4 antibody at 1/1,000 dilution. Positive control:
Lane 1: SW480
Lane 2: SKOV-3
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-HE4 antibody. Counter stained with hematoxylin.
ICC staining HE4 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining HE4 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SW480 cells with HE4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
HE4 (whey acidic protein (WAP)-type four-disulfide core-2, WFDC2) is a small secretory protein that may influence sperm maturation. HE4 gene expression is high in pulmonary epithelial cells and in some ovarian cancers. HE4 protein has a WAP motif that contains eight cysteines forming four disulfide bonds at the core of the protein. The WAP motif functions as a protease inhibitor in many of the family members that contain them.

If you have published an article using product 49375, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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