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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Jagged1 Rabbit mAb

Jagged1 Rabbit mAb#49377

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Product Detail

Product NameJagged1 Rabbit mAb

Clone No.JF96-06

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB IHC ICC/IF

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAGS antibody
AHD antibody
AWS antibody
CD 339 antibody
CD339 antibody
CD339 antigen antibody
Headturner antibody
hJ1 antibody
Htu antibody
Jag 1 antibody
Jag1 antibody
JAG1_HUMAN antibody
Jagged 1 antibody
Jagged1 (Alagille syndrome) antibody
Jagged1 antibody
JAGL1 antibody
MGC104644 antibody
OTTHUMP00000030278 antibody
Protein jagged-1 antibody
Ser 1 antibody
Ser1 antibody
Serrate 1 antibody
Slalom antibody

Accession NoSwiss-Prot#:P78504

Uniprot P78504

Gene ID 182;

Calculated MWPredicted band size: 134 kDa

Sdspage MWObserved band size: 180 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
All lanes: Jagged1 Rabbit mAb at 1/1k dilution

Lane 1 : HepG2 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 134 kDa
Observed band size: 180 kDa

Exposure time: 13 seconds
All lanes :Jagged1 Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 : Jagged1 knockdown HT-1080 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human kidney tissue stained for Jagged1 using 49377 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Jagged1 using 49377 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Jagged1 antibody (49377)

ICC/IF staining of Jagged1 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 49377 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

The LIN-12/Notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. Ligands for Notch include Jagged1, Jagged2 and Delta. Jagged is a membrane protein and can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. It is involved in mammalian cardiovascular development and in cell-fate decisions during hematopoiesis. Jagged is expressed in adult and fetal tissues, and expression is upregulated in cervical squamous cell carcinoma. Familial Tetralogy of Fallot, the most common form of complex congenital heart disease, is caused by a mutation in the Jagged1 gene.

If you have published an article using product 49377, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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