Product Detail
Product NameHuman IgM Rabbit mAb
Clone No.SR4540
Host SpeciesRecombinant Rabbit
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB;IHC;ICC/IF;IP;FC
Species ReactivityHu
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesImmunoglobin heavy chain constant region mu edit item name - Immunoglobin heavy chain mu constant region antibody Immunoglobin heavy chain constant region mu antibody AGM1 antibody Constant region of heavy chain of IgM antibody DKFZp686I15196 antibody DKFZp686I15212 antibody FLJ00385 antibody Ig mu chain C region antibody IGHM antibody IgM heavy chain constant region antibody Immunoglobin heavy constant mu antibody Immunoglobulin mu antibody MGC104996 antibody MGC52291 antibody MU antibody VH antibody
Accession NoSwiss-Prot#:P01871
Uniprot
P01871
Calculated MW75 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
Western blot analysis of Human IgM on human plasma lysates using anti-Human IgM antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Human IgM antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Human IgM antibody. Counter stained with hematoxylin.
Immunoglobulin M (IgM) is the largest circulating antibody molecule in humans. It consists of a heavy chain (?-chain) and a light chain (κ- or λ-chain), as well as 5 base units and 10 binding sites, though it cannot bind all 10 simultaneously because of steric hindrance. IgM chain C refers to the constant region of the IgM heavy chain that is involved in immune regulation. IgM forms polymers by covalently linking multiple immunoglobulins together with disulfide bonds. It normally exists as a pentamer, but occasionally as a hexamer. Because of its polymeric nature, IgM has high avidity, and it is especially effective at complement activation. Due to its large size, IgM does not diffuse well, and it is found in the interstitium in very low amounts. IgM is mainly found in serum; however, because of the J chain, it is also important as a secretory immunoglobulin. IgM is the first immunoglobulin expressed by mature B cells, and it normally appears early in the course of an infection and does not reappear after further exposure.
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