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Gli1 Rabbit mAb#49399

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Product Detail

Product NameGli1 Rabbit mAb

Clone No.JF09-08

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesGli 1 antibody
GLI antibody
GLI family zinc finger 1 antibody
GLI Kruppel family member 1 antibody
gli1 antibody
GLI1_HUMAN antibody
Glioma associated oncogene 1 antibody
Glioma associated oncogene homolog 1 (zinc finger protein) antibody
Glioma associated oncogene homolog antibody
Glioma-associated oncogene antibody
Oncogene GLI antibody
Zfp 5 antibody
Zfp5 antibody
Zinc finger protein GLI 1 antibody
Zinc finger protein GLI1 antibody

Accession NoSwiss-Prot#:P08151

Uniprot P08151

Gene ID 2735;

Calculated MW150 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1,000
IHC:1:50-1:200
ICC: 1:50-1:200

Western blot analysis of Gli1 on SH-SY-5Y cells lysates using anti-Gli1 antibody at 1/1,000 dilution.
ICC staining Gli1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Gli1 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Gli1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. GLI-1 (GLI family zinc finger 1), also known as Glioma-associated oncogene or oncogene GLI, is a 1,106 amino acid protein that localizes to both the cytoplasm and nucleus, and belongs to the GLI C2H2-type zinc-finger protein family. GLI-1 acts as a transcriptional activator and is thought to play a role in craniofacial development. GLI-1 exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 12q13.3.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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