Product Detail
Product NameThioredoxin Rabbit mAb
Clone No.JF101-8
Host SpeciesRecombinant Rabbit
Clonality Monoclonal
PurificationProA affinity purified
ApplicationsWB, FC
Species ReactivityHu, Ms, Rt
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesADF antibody
ATL derived factor antibody
ATL-derived factor antibody
DKFZp686B1993 antibody
MGC61975 antibody
SASP antibody
Surface associated sulphydryl protein antibody
Surface-associated sulphydryl protein antibody
testicular tissue protein Li 199 antibody
THIO_HUMAN antibody
Thioredoxin antibody
thioredoxin delta 3 antibody
TRDX antibody
TRX 1 antibody
Trx antibody
TRX1 antibody
TXN antibody
TXN delta 3 antibody
TXN protein antibody
zgc:92903 antibody
Accession NoSwiss-Prot#:P10599
Uniprot
P10599
Gene ID
7295;
Calculated MW12 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-5,000
FC: 1:50-1:100
Western blot analysis of TRX1 on different lysates using anti-TRX1 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: 293T
Flow cytometric analysis of Hela cells with TRX1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Thioredoxin (Trx) is a redox protein that is found in several species, such as bacteria, plants and mammals, and contains a conserved active site, consisting of Trp-Cys-Gly-Pro-Cys. Trx has several biological functions. It acts as a hydrogen donor for ribonucleotide reductase, which is critical for DNA synthesis, and modulates the DNA-binding activity of several transcription factors, including NFkB, AP-1, p53, TFIIIC and glucocorticoid receptor. Trx also stimulates cell growth, is an inhibitor of apoptosis and plays a role in the protection against oxidative stress. Drugs that inhibit Trx have antitumor activity, suggesting that Trx is involved in a variety of human diseases, including cancer. TrxR is a ubiquitously expressed flavoprotein that catalyzes the NADPH-dependent reduction of Trx as well as several other oxidized cellular components.
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