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Ras Rabbit mAb#49408

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Product Detail

Product NameRas Rabbit mAb

Clone No.JF10-11

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IP

Species ReactivityHu, Ms, Rt, zebrafish

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesC-BAS/HAS antibody
c-H-ras antibody
C-HA-RAS1 antibody
CTLO antibody
GTPase HRas antibody
GTPase KRas antibody
GTPase NRas antibody
H-Ras-1 antibody
H-RASIDX antibody
Ha-Ras antibody
HAMSV antibody
HRAS antibody
HRAS1 antibody
K RAS2A antibody
K RAS2B antibody
K RAS4A antibody
K RAS4B antibody
K-RAS antibody
KRAS antibody
KRAS1 antibody
KRAS2 antibody
N-RAS antibody
N-terminally processed antibody
NRAS antibody
NRAS1 antibody
p21ras antibody
RASH_HUMAN antibody
RASH1 antibody
RASK2 antibody
Transforming protein p21 antibody
v Ha ras Harvey rat sarcoma viral oncogene homolog antibody
v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody
v ras neuroblastoma RAS viral oncogene homolog antibody

Accession NoSwiss-Prot#:P01111

Uniprot P01111

Gene ID 4893;

Calculated MW21 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000

ICC: 1:100-1:500

Western blot analysis of Ras on different lysates using anti-Ras antibody at 1/1,000 dilution. Positive control:
Lane 1: 293T
Lane 2: MCF-7
Lane 3: Hela
Lane 4: zebrafish
ICC staining Ras in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Ras in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Ras in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
The mammalian c-H-, c-K- and N-Ras proto-oncogenes encode guanine nucleotide-binding proteins that are ubiquitously expressed in vertebrate cells. c-H- and c-K-Ras are cellular homologs of the v-H and v-K-Ras sequences originally isolated from the Harvey and Kirsten strains of rat sarcoma virus. Ras-encoded proteins bind GDP and GTP with high affinity and possess a low level intrinsic GTPase activity that can be stimulated over 100-fold by interaction with cytosolic GTPase activating protein (GAP), a potential effector for Ras p21 function. Point mutations at amino acids 12, 13, 59 and 61 within domains responsible for GTP binding and hydrolysis activate Ras proteins to their oncogenic form and block the ability of the GTPase activity to be stimulated by GAP. Several additional proteins with GAP activity have been identified and shown to interact with p21 Ras or other members of the Ras gene family.

If you have published an article using product 49408, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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