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C3 Rabbit mAb#49413

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Product Detail

Product NameC3 Rabbit mAb

Clone No.SR4382

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF, FC

Species ReactivityHu

Immunogen DescA synthesized peptide derived from human Complement C3

ConjugateUnconjugated

Other NamesAcylation stimulating protein cleavage product antibody
AHUS5 antibody
ARMD9 antibody
ASP antibody
C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
C3 antibody
CO3_HUMAN antibody
Complement C3 antibody
Complement C3c alpha'' chain fragment 2 antibody
Complement component 3 antibody
Complement factor 3 antibody
CPAMD1 antibody
HEL S 62p antibody

Accession NoSwiss-Prot#:P01024

Uniprot P01024

Gene ID 718;

Calculated MW187 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Application Details
WB 1:1000-1:2000
ICC/IF 1:50-1:200
FC 1:20-1:100
ICC staining C3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of HepG2 cells with C3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of C3 / C3a expression in Human plasma lysate.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

If you have published an article using product 49413, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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