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Location： Home > Recombinant Rabbit Monoclonal Antibodies > SMC3 Rabbit mAb

SMC3 Rabbit mAb#49448

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Product Detail

Product NameSMC3 Rabbit mAb

Clone No.JM10-75

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesBAM antibody
Bamacan antibody
Basement membrane associated chondroitin proteoglycan antibody
Basement membrane-associated chondroitin proteoglycan antibody
BMH antibody
CDLS3 antibody
chondroitin sulfate proteoglycan 6 (bamacan) antibody
Chondroitin sulfate proteoglycan 6 antibody
Chromosome associated polypeptide antibody
Chromosome-associated polypeptide antibody
CSPG 6 antibody
CSPG6 antibody
hCAP antibody
Human chromosome associated polypeptide antibody
im:7142991 antibody
SMC 3 antibody
SMC protein 3 antibody
SMC-3 antibody
smc3 antibody
SMC3_HUMAN antibody
SMC3L1 antibody
Structural maintenance of chromosome 3 antibody
Structural maintenance of chromosomes 3 antibody
Structural maintenance of chromosomes protein 3 antibody
u:fb22e01 antibody
wu:fc30d07 antibody

Accession NoSwiss-Prot#:Q9UQE7

Uniprot Q9UQE7

Gene ID 9126;

Calculated MW141 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100
Western blot analysis of SMC3 on different cells lysates using anti-SMC3 antibody at 1/1,000 dilution. Positive control:
Line1: HepG2
Line2: NIH-3T3
Line3:PC12
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SMC3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SMC3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SMC3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SMC3 antibody. Counter stained with hematoxylin.
ICC staining SMC3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining SMC3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining SMC3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with SMC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis. The two distinct classes of SMC protein complexes are comprised of SMC1 (also designated SB1.8) with SMC3 (also designated HCAP for human chromosome-associated protein and Bamacan for the secreted proteoglycan), and SMC2 (also designated hCAP-E) with SMC4 (also designated hCAP-C). The SMC1/SMC3 complex is required for metaphase progression in mitotic cells and functions independently of the SMC2/SMC4 complex during the cell cycle. SMC1 is ubiqitiously expressed in various human tissues, including thymus, testis, and colon. SMC3 is expressed as a nuclear protein in the colon, but can also occur as a secreted proteoglycan expressed in testis and brain. The secreted proteoglycan contains several glycosylation sites and is thought to play a role in basement membrane physiology.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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