Product Detail
Product NameTIA1 Rabbit mAb
Clone No.JM42-11
Host SpeciesRecombinant Rabbit
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF, IHC, IP
Species ReactivityHu, Ms
Immunogen Descrecombinant protein
ConjugateUnconjugated
Other NamesCytotoxic granule associated RNA binding protein 1 antibody
Cytotoxic granule associated RNA binding protein antibody
mTIA-1 antibody
Nucleolysin TIA 1 isoform p40 antibody
Nucleolysin TIA-1 isoform p40 antibody
Nucleolysin TIA1 isoform p40 antibody
p40 TIA 1 antibody
p40-TIA-1 (containing p15-TIA-1) antibody
p40-TIA-1 antibody
RNA binding protein TIA 1 antibody
RNA binding protein TIA1 antibody
RNA-binding protein TIA-1 antibody
T-cell-restricted intracellular antigen-1 antibody
TIA 1 antibody
TIA 1 cytotoxic granule associated RNA binding protein antibody
Tia antibody
TIA-1 antibody
TIA1 antibody
TIA1 cytotoxic granule associated RNA binding protein antibody
TIA1 cytotoxic granule associated RNA binding protein like 1 antibody
TIA1 protein antibody
TIA1_HUMAN antibody
TIAL1 antibody
TIAR antibody
WDM antibody
Accession NoSwiss-Prot#:P31483
Uniprot
P31483
Gene ID
7072;
Calculated MW43 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:10-1:50
Western blot analysis of TIA1 on Jurkat cells lysates using anti-TIA1 antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TIA1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TIA1 antibody. Counter stained with hematoxylin.
ICC staining TIA1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining TIA1 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining TIA1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
FAS, also referred to as CD95 or APO-1, is a type I transmembrane protein that plays a central role mediating viral immunity. TIA-1 and TIAR are two closely related proteins that possess three RRMs (RNA recognition motifs), designated RRM 1, 2 and 3. Although both TIA-1 and TIAR are thought to function as mediators of apoptotic cell death, their specific roles in such pathways are unknown. Unlike TIA-1, which is found in the granules of cytotoxic lymphocytes, TIAR expression is limited to the nucleus and found in a much broader range of cells including, but not limited to, cells of hematopoietic origin. TIAR is translocated to the cytoplasm shortly after FAS ligation and this event immediately proceeds the onset of DNA fragmentation. A novel serine/threonine kinase that is activated as a result of FAS ligation, designated FAST (FAS-activated serine/threonine), shows kinase specificity towards both TIA-1 and TIAR. In unstimulated Jurkat cells, FAST resides in the cytoplasm as a highly phosphorylated protein and is quickly dephosphorylated and activated in response to stimulated FAS.
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