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CRISPR-Cas9 SP Rabbit mAb#49498

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Product NameCRISPR-Cas9 SP Rabbit mAb

Clone No.JM11-55

ClonalityMonoclonal

PurificationAffinity-chromatography

ApplicationsWB,IHC,ICC/IF,FC

Species ReactivityRecombinant Cas9 Streptococcus pyogenes

Immunogen DescRecombinant fragment derived from Streptococcus pyogenes

ConjugateUnconjugated

Other NamesCas9 antibody
CRISPR-associated endonuclease Cas9/Csn1 antibody
CRISPR-Cas9/Csn1 antibody
csn1 antibody
SpyCas9 antibody

Accession NoSwiss-Prot#:Q99ZW2

Uniprot Q99ZW2

Gene ID 901176;

Calculated MW158 kDa

Concentration0.6mg/ml

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Application Details

WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50

Western blot analysis of CRISPR-Cas9 SP expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate.
Immunofluorescent analysis of 293T cells transfected with CRISPR-SpCas9, using CRISPR-Cas9 SP Antibody .

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

If you have published an article using product 49498, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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