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mSin3A Rabbit mAb#49594

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Product Detail

Product NamemSin3A Rabbit mAb

Clone No.JA94-31

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAW553200 antibody
DKFZP434K2235 antibody
FLJ90319 antibody
Histone deacetylase complex subunit Sin 3a antibody
Histone deacetylase complex subunit Sin3a antibody
KIAA0700 antibody
Kiaa4126 antibody
mKIAA4126 antibody
Paired amphipathic helix protein Sin 3a antibody
Paired amphipathic helix protein Sin3a antibody
Sin 3a antibody
SIN3 homolog A antibody
SIN3 homolog A transcription regulator (yeast) antibody
SIN3 homolog A transcription regulator antibody
SIN3 transcription regulator homolog A antibody
Sin3a antibody
SIN3A protein antibody
SIN3A_HUMAN antibody
Transcriptional co repressor Sin 3A antibody
Transcriptional co repressor Sin3A antibody
Transcriptional corepressor Sin3a antibody
Transcriptional regulator SIN3A antibody

Accession NoSwiss-Prot#:Q96ST3

Uniprot Q96ST3

Gene ID 25942;

Calculated MW145 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of mSin3A on 293T cell using anti-mSin3A antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-mSin3A antibody. Counter stained with hematoxylin.
ICC staining mSin3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining mSin3A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining mSin3A in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of MCF-7 cells with mSin3A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form heterodimers with at least four related proteins designated Mad 1, Mxi1 (alternatively designated Mad 2), Mad 3 and Mad 4. Like Mad 1 and Mxi1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated, while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad-binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2). It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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