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Placental alkaline phosphatase Rabbit mAb#49615

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Product Detail

Product NamePlacental alkaline phosphatase Rabbit mAb

Clone No.JM22-53

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesAlkaline phosphatase antibody
Alkaline phosphatase placental antibody
Alkaline phosphatase placental type antibody
Alkaline phosphatase Regan isozyme antibody
ALP antibody
Alp1 antibody
ALPP antibody
FLJ61142 antibody
Germ-cell alkaline phosphatase antibody
nagao isozyme antibody
OTTHUMP00000164354 antibody
PALP antibody
Placental alkaline phosphatase 1 antibody
placental heat-stable alkaline phosphatase antibody
placental type antibody
PLAP antibody
PLAP-1 antibody
PLAP1 antibody
PPB1_HUMAN antibody

Accession NoSwiss-Prot#:P05187

Uniprot P05187

Gene ID 250;

Calculated MW70 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of PLAP on human placenta tissue (1) and Hela cell (2) lysate using anti-PLAP antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PLAP antibody. Counter stained with hematoxylin.
ICC staining PLAP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PLAP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alkaline phosphatases (AP) are glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+-metallated glycoproteins that catalyze the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. Placental alkaline phosphatase (also known as PLAP, ALPP, PALP, placental ALP-1 or Regan isozyme) is a 530 amino acid, tissue-specific AP that is expressed in the placenta, the serum of pregnant women and ectopically expressed in various cancers, including those of the ovary and testis. PLAP may assist in guiding migratory cells and transporting specific molecules, such as fatty acids and immunoglobulins, across the plasma membrane. The three tissue-specific APs identified in human, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous and their genes are clustered on chromosome 2q.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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