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ETS1 Rabbit mAb#49617

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Product Detail

Product NameETS1 Rabbit mAb

Clone No.JM92-32

Host SpeciesRecombinant Rabbit

Clonality Monoclonal

PurificationProA affinity purified

ApplicationsWB, IHC, FC

Species ReactivityHu, Ms

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesAvian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
C ets 1 protein antibody
c-ets-1 antibody
ETS 1 antibody
Ets protein antibody
ETS proto-oncogene 1, transcription factor antibody
ETS1 antibody
ETS1 oncogene antibody
ETS1 protein antibody
ETS1_HUMAN antibody
EWSR 2 antibody
EWSR2 antibody
FLJ10768 antibody
Oncogene ETS1 antibody
P54 antibody
Protein C-ets-1 antibody
v ets avian erythroblastosis virus E2 oncogene homolog antibody
v ets avian erythroblastosis virus E2 oncogene homolog 1 antibody
v ets avian erythroblastosis virus E26 oncogene homolog 1 antibody
v ets erythroblastosis virus E26 oncogene homolog 1 antibody
v-ets erythroblastosis virus E26 oncogene homolog 1 antibody

Accession NoSwiss-Prot#:P14921

Uniprot P14921

Gene ID 2113;

Calculated MW50 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200


FC: 1:50-1:100
Western blot analysis of ETS1 on different cell using anti-ETS1 antibody at 1/1,000 dilution. Positive control��
Line1: Jurkat
Line2: K562
Line3: HepG2
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ETS1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ETS1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-ETS1 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Jurkat cells with ETS1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element. Evidence indicates that the DNA binding activity by Ets-1 is regulated at the level of phosphorylation.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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