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CD31 Rabbit mAb#52064

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Product Detail

Product NameCD31 Rabbit mAb

Clone No.S04-2F3

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity Purified

ApplicationsWB

Species ReactivityHuman

Immunogen DescA synthetic peptide of human CD31

ConjugateUnconjugated

Other NamesCD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM

Accession NoSwiss-Prot:P16284
GeneID:5175

Uniprot P16284

Gene ID 5175

Calculated MWCalculated MW: 83 kDa; Observed MW: 130 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1/1000;
Western blot detection of CD31 in Jurkat,3T3 cell lysates using CD31 Rabbit mAb(1:1000 diluted).Predicted band size:83kDa.Observed band size:130kDa.
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions . Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes .

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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