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c Jun (Phospho-Ser63) Rabbit mAb#52101

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Product Namec Jun (Phospho-Ser63) Rabbit mAb

Clone No.S07-3G7

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity Purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic phosphopeptide corresponding to residues surrounding Ser63 of human c-Jun

ConjugateUnconjugated

Other NamesAH119; AP1; Activator protein 1; Jun A; c-Jun;

Accession NoSwiss-Prot:P05412
GeneID:3725

Uniprot P05412

Gene ID 3725

Calculated MWPredicted band size: 36 kDa

Sdspage MWObserved band size: 48 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : c Jun (Phospho-Ser63) Rabbit mAb at 1/1k dilution
Lane 1 : 3T3 whole cell lysates
Lane 2 : C6 whole cell lysates
Lane 3 : K562 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 48 kDa

Exposure time: 3 seconds
All lanes : c Jun (Phospho-Ser63) Rabbit mAb at 1/1k dilution
Lane 1 : 3T3 whole cell lysates
Lane 2 : 3T3 treated with 5ug/ml Anisomycin for 30 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 48kDa

Exposure time: 5 seconds
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for c Jun (Phospho-Ser63) using 52101 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat liver carcinoma tissue stained for c Jun (Phospho-Ser63) using 52101 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence c Jun (Phospho-Ser63) antibody (52101)

ICC/IF staining of c Jun (Phospho-Ser63) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 52101 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Swiss-Prot Acc.P05412.Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' (PubMed:10995748, PubMed:22083952). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity (By similarity). Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed:12618758). Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (PubMed:17210646).

If you have published an article using product 52101, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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