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Adipose Triglyceride Lipase Rabbit mAb#52124

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Product Detail

Product NameAdipose Triglyceride Lipase Rabbit mAb

Clone No.S08-3F5

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity Purified

ApplicationsWB

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic peptide of human Adipose Triglyceride Lipase

ConjugateUnconjugated

Other NamesATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik

Accession NoSwiss-Prot:Q96AD5
GeneID:57104

Uniprot Q96AD5

Gene ID 57104

Calculated MWCalculated MW: 55 kDa; Observed MW: 55 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1/1000
Western blot detection of Adipose Triglyceride Lipase in K562 cell lysates using Adipose Triglyceride Lipase Rabbit mAb(1:500 diluted).Predicted band size:55kDa.Observed band size:55kDa.
All lanes : ATGL Rabbit mAb at 1/1k dilution

Lane 1 : 3T3 whole cell lysates
Lane 2 : C6 whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 55 kDa
Observed band size: 55 kDa

Exposure time: 5 seconds
All lanes : ATGL Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : ATGL knockout HAP1 cell lysate

Lysates/proteins at 20 µg per lane.
Swiss-Prot Acc.Q96AD5.Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed:15550674). Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes (PubMed:16239926). May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.

If you have published an article using product 52124, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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