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Transcription Factor AP 2 gamma Rabbit mAb#52147

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Product NameTranscription Factor AP 2 gamma Rabbit mAb

Clone No.S03-8I2

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity Purified

ApplicationsWB IF

Species ReactivityHuman

Immunogen DescRecombinant protein of human AP2 gamma

ConjugateUnconjugated

Other NamesERF1; TFAP2G; hAP-2g; AP2-GAMMA

Accession NoSwiss-Prot:Q92754
GeneID:7022

Uniprot Q92754

Gene ID 7022

Calculated MWCalculated MW: 49 kDa; Observed MW: 49 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1/1000; ICC/IF: 1/50;
Western blot detection of TFAP2C/AP2 gamma in Jurkat,Hela cell lysates using TFAP2C/AP2 gamma Rabbit mAb(1:500 diluted).Predicted band size:49kDa.Observed band size:49kDa.
Immunofluorescence of TFAP2C/AP2 gamma (green) in MCF-7 using TFAP2C/AP2 gamma antibody at dilution 1/20, and DAPI(blue)
Swiss-Prot Acc.Q92754.Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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