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CDKN2A Rabbit mAb#52214

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Product Detail

Product NameCDKN2A Rabbit mAb

Clone No.S04-8C9

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity Purified

ApplicationsWB IF

Species ReactivityHuman,Mouse

Immunogen DescRecombinant protein of human CDKN2A/p16INK4a

ConjugateUnconjugated

Other NamesARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A

Accession NoSwiss-Prot:P42771
GeneID:1029

Uniprot P42771

Gene ID 1029

Calculated MWCalculated MW: 17 kDa; Observed MW: 17 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1/1000; ICC/IF: 1/20-1/100;
Western blot detection of CDKN2A/p16INK4a in 293 cell lysates using CDKN2A/p16INK4a Rabbit mAb(1:1000 diluted).Predicted band size:17KDa.Observed band size:17KDa.
Western blot detection of CDKN2A-p16INK4a in 3T3,Hela cell lysates using CDKN2A/p16INK4a Rabbit mAb(1:1000 diluted).Predicted band size:17KDa.Observed band size:17KDa.
Immunofluorescence of CDKN2A/p16INK4a(green) in Hela cells using CDKN2A/p16INK4a Rabbit mAb at dilution 1/5, and DAPI(blue)
Swiss-Prot Acc.P42771.Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

If you have published an article using product 52214, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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