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TRIM56 Rabbit mAb#52633

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Product Detail

Product NameTRIM56 Rabbit mAb

Clone No.S02-8A3

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity Purified

ApplicationsWB IF

Species ReactivityHuman

Immunogen DescA synthetic peptide of human TRIM56

ConjugateUnconjugated

Other NamesRING finger protein 109; RNF109

Accession NoSwiss-Prot:Q9BRZ2
GeneID:81844

Uniprot Q9BRZ2

Gene ID 81844

Calculated MWCalculated MW: 82 kDa; Observed MW: 82 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1/2000-1/10000; ICC/IF: 1/50;
Western blot detection of TRIM56 in Hela cell lysates using TRIM56 Rabbit mAb(1:1000 diluted).Predicted band size:82kDa.Observed band size:82kDa.
Immunocytochemistry of TRIM56(green) in Hela cells using TRIM56 Rabbit mAb at dilution 1/50, and DAPI(blue)
Swiss-Prot Acc.Q9BRZ2.E3 ubiquitin-protein ligase that plays a key role in innate antiviral immunity (PubMed:21289118). In response to pathogen- and host-derived double-stranded DNA (dsDNA), targets TMEM173/STING to 'Lys-63'-linked ubiquitination, thereby promoting its homodimerization, a step required for the production of type I interferon IFN-beta . Independently of its E3 ubiquitin ligase activity, positive regulator of TLR3 signaling. Potentiates extracellular double stranded RNA (dsRNA)-induced expression of IFNB1 and interferon-stimulated genes ISG15, IFIT1/ISG56, CXCL10, OASL and CCL5/RANTES. Promotes establishment of an antiviral state by TLR3 ligand and TLR3-mediated chemokine induction following infection by hepatitis C virus (PubMed:22948160).

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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