Product Detail
Product NameYes1 Rabbit mAb
Clone No.S07-8B3
Host SpeciesRecombinant Rabbit
ClonalityMonoclonal
IsotypeRabbit IgG
PurificationAffinity Purified
ApplicationsWB
Species ReactivityHuman
Immunogen DescA synthetic peptide of human Yes1
ConjugateUnconjugated
Other NamesYes; c-yes; HsT441; P61-YES
Accession NoSwiss-Prot:P07947
GeneID:7525
Uniprot
P07947
Gene ID
7525
Calculated MWCalculated MW: 61 kDa; Observed MW: 61 kDa
Concentration0.3 mg/ml
Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.
Application Details
WB: 1/1000-1/5000;
Western blot detection of Yes1 in K562 cell lysates using Yes1 Rabbit mAb(1:1000 diluted).Predicted band size:61kDa.Observed band size:61kDa.
Swiss-Prot Acc.P07947.Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis.
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Yes
