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BUBR1 Rabbit mAb#52727

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Product Detail

Product NameBUBR1 Rabbit mAb

Clone No.S05-3G7

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeIgG

PurificationAffinity Purified

ApplicationsWB IHC

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic peptide of human BubR1

ConjugateUnconjugated

Other NamesMVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta

Accession NoSwiss-Prot:O60566
GeneID:701

Uniprot O60566

Gene ID 701

Calculated MWCalculated MW:120 kDa,Observed MW:120 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1/2000-1/10000 IHC: 1/20
Western blot detection of BubR1 in CHO-K1,Ramos cell lysates using BubR1 Rabbit mAb(1:1000 diluted).Predicted band size:120kDa.Observed band size:120kDa.
Immunohistochemistry of BubR1 in paraffin-embedded Human lung cancer tissue using BubR1 Rabbit mAb at dilution 1/50
Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

If you have published an article using product 52727, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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