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Histone H2A.X Rabbit mAb#52773

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Product NameHistone H2A.X Rabbit mAb

Clone No.S02-3C8

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeIgG

PurificationAffinity Purified

ApplicationsWB IHC

Species ReactivityHuman

Immunogen DescA synthetic peptide of human Histone H2A.X

ConjugateUnconjugated

Other NamesH2A.X; H2A/X; H2AFX

Accession NoSwiss-Prot:P16104
GeneID:3014

Uniprot P16104

Gene ID 3014

Calculated MWCalculated MW:15 kDa,Observed MW:15 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1/1000 IHC: 1/50
Western blot detection of Histone H2A.X in Hela,Ramos using Histone H2A.X Rabbit mAb(1:1000 diluted)
Immunohistochemistry of Histone H2A.X in paraffin-embedded Human lung cancer tissue using Histone H2A.X Rabbit mAb at dilution 1/50
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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