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PI3 Kinase p85 alpha Rabbit mAb#52838

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Product Detail

Product NamePI3 Kinase p85 alpha Rabbit mAb

Clone No.S08-8H6

Host SpeciesRecombinant Rabbit

ClonalityMonoclonal

IsotypeIgG

PurificationAffinity Purified

ApplicationsWB

Species ReactivityHuman,Mouse,Rat

Immunogen DescA synthetic peptide of human PI 3 Kinase p85 alpha

ConjugateUnconjugated

Other Namesp85; AGM7; GRB1; IMD36; p85-ALPHA

Accession NoSwiss-Prot:P27986
GeneID:5295

Uniprot P27986

Gene ID 5295

Calculated MWCalculated MW:84 kDa,Observed MW:84 kDa

Concentration0.3 mg/ml

Formulation50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

StorageStore at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

Application Details
WB: 1/1000
Western blot detection of PI 3 Kinase p85 alpha in HL-60,U2OS,C6 cell lysates using PI 3 Kinase p85 alpha Rabbit mAb(1:1000 diluted).Predicted band size:84kDa.Observed band size:84kDa.
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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