Yes
All lanes: GRP78 BiP Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa
Exposure time: 6 seconds
All lanes: GRP78 BiP Rabbit mAb at 1/1k dilution
Lane 1 : Mouse brain lysates
Lane 2 : Mouse liver lysatesLane 3 : Rat brain lysatesLane 4 : Rat liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa
Exposure time: 8 seconds
All lanes:GRP78 BiP Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type 293T cell lysate
Lane 2 : GRP78 BiP knockdown 293T cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human testis tissue stained for GRP78 BiP using 56022 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat testis tissue stained for GRP78 BiP using 56022 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence GRP78 BiP antibody (56022) ICC/IF staining of GRP78 BiP in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56022 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
